Journal: International Journal of Oncology
Article Title: PP2B and ERK1/2 regulate hyaluronan synthesis of HT168 and WM35 human melanoma cell lines
doi: 10.3892/ijo.2015.3313
Figure Lengend Snippet: Detection of hyaluronic acid, HAS2 and HAS3 in normal healthy tissues and malignant metastases. Row 1, normal skin, 2, mesenteric lymph node, 3, melanoma metastasis from mesenteric lymph node, 4, normal lung, 5, melanoma metastasis from lung. (A) HA affinity histochemistry. (B) HAS2. (C) HAS3 immunohistochemistry. HA is labelled with Streptavidin-Alexa 555 (red), HAS2 and HAS3 anti-rabbit-Alexa 555 (red). Melanocytes and melanoma cells are shown with MelanA-Alexa 488 (green). Nuclei are demonstrated with DAPI (blue). Scale bar, 50 μm. Representative photomicrographs of 3 independent experiments are shown.
Article Snippet: In cadaver tissue samples, monoclonal MelanA antibody (Novocastra Laboratories Ltd., Newcastle, UK) was used to demonstrate melanin-positive cells labelling with an Alexa 488 conjugated anti-mouse antibody (Invitrogen Corporation) at a dilution 1:1,000.
Techniques: Immunohistochemistry